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Recombinant Murine M-CSF
340 人阅读发布时间：2019-09-19 10:18
Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM
Description: M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts,
endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood
monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating
dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates
hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule
exhibits no activity on human cells.--Recombinant Murine M-CSF is a 36.4 kDa homodimeric protein consisting of two 156 amino
acid polypeptide subunits.
Sequence-(monomer):-MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER
LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
Storage & Handling:
Reconstitution: Centrifuge vial prior to opening. Reconstitute in water to 0.1-0.5 mg/ml. Do not vortex. Store at 2°C
to 8°C for 1 week, or prepare for extended storage.
Extended Storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA).
Store working aliquots at -20˚C to -80˚C. Avoid repeated freeze-thaw cycles.
Storage/Stability: Product Form Temperature Storage Time
Lyophilized -20˚ to -80˚C December 2023
Lyophilized 4°C 6 months
Lyophilized Room Temperature 1 month
Reconstituted 2°C to 8°C 1 week
Extended Storage -20˚ to -80˚C 12 months
Specifications:
Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized from 5mM Sodium Phosphate, pH 8.0 +
100mM NaCl.
Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (< 1 EU/μg).
Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.
Biological Activity: Determined by its ability to stimulate the proliferation of murine M-NFS-60 cells. The expected-ED50-is
≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106-units/mg.
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