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Recombinant Murine TNF-α
174 人阅读发布时间：2019-09-18 11:27
Synonyms: Tumor Necrosis Factor-α, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin, TNFSF1A
Description: TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes,
macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1
and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against
bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis,
inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms;
a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolytically cleaved to yield
a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution.--Recombinant Murine
TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length
transmembrane protein.
Sequence:-MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK
VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Storage & Handling:
Reconstitution: Centrifuge vial prior to opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. Store at 2°C
to 8°C for 1 week, or prepare for extended storage.
Extended Storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA).
Store working aliquots at -20˚C to -80˚C. Avoid repeated freeze-thaw cycles.
Storage/Stability: Product Form Temperature Storage Time
Lyophilized -20˚ to -80˚C December 2021
Lyophilized 4°C 6 months
Lyophilized Room Temperature 1 month
Reconstituted 2°C to 8°C 1 week
Extended Storage -20˚ to -80˚C 12 months
Specifications:
Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5.
Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (< 1 EU/μg).
Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.
Biological Activity: Determined by the cytolysis of murine L929 cells in the presence of Actinomycin D. The expected-ED50-
is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107-units/mg.
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